A New Enzymatic Method for Assaying Serum Inorganic Pyrophosphatase.
نویسندگان
چکیده
منابع مشابه
Enzymatic method for assaying calcium in serum and urine with porcine pancreatic alpha-amylase.
We developed a kinetic assay for calcium in serum and urine, based on the activation of porcine pancreatic alpha-amylase (EC 3.2.1.1) with 2-chloro-4-nitrophenyl-alpha-maltotrioside as substrate. The kinetic generation of 2-chloro-4-nitrophenol, monitored at 405 nm, was proportional to the concentration of calcium in serum and urine. The assay was developed and evaluated with the Cobas Bio cent...
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متن کاملYeast Inorganic Pyrophosphatase
The kinetics of the Mg2+ activation of crystalline yeast inorganic pyrophosphatase have been thoroughly investigated from pH 7.40 to pH 9.05 using a sensitive isotope assay in order to determine the role of the divalent metal ion activator in the reaction. A computer program has been devised for calculating the concentration of each of the various components of the complex equilibrium involving...
متن کاملYeast Inorganic Pyrophosphatase
The magnesium ion-dependent hydrolysis of pyrophosphate catalyzed by yeast inorganic pyrophosphatase is markedly inhibited by calcium ion. The kinetics of inhibition have been measured and analyzed in terms of a model involving strong interactions between CaPPi and two metalcomplexed forms of the enzyme, EMg and ECa. Inhibition constants for these interactions have been estimated by nonlinear r...
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ژورنال
عنوان ژورنال: Journal of Clinical Biochemistry and Nutrition
سال: 1998
ISSN: 1880-5086,0912-0009
DOI: 10.3164/jcbn.25.1